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New Web TV Player. The video is not stored on our server. Page 1 Page 2 Page 3 Page 4. EAFYS G.B. Khan Video Tutorial.Polyphenols from the leaves of Lagerstroemia speciosa suppress the expression of alpha-synuclein and tyrosine hydroxylase in PC12 cells. Recently, several lines of evidence showed that the accumulation and aggregation of alpha-synuclein (alpha-syn) in the neurons are a key step in the progression of Parkinson's disease. Tyrosine hydroxylase (TH), which is the rate-limiting enzyme in catecholamine synthesis, is another protein that has been shown to have a strong association with Parkinson's disease. In the present study, the chemical composition of an ethanolic extract of the leaves of Lagerstroemia speciosa (LS), the only Malay lagerstroeme tea cultivar, was investigated using GC-MS. Twenty-one compounds, representing 94.5% of the total extracts, were identified and, among them, lagerstroemin and its derivatives were detected as the major constituents. The in vitro antioxidant activity of the extract was evaluated using 1,1-diphenyl-2-picrylhydrazyl, total antioxidant capacity and ferric reducing antioxidant power assays, and the results showed that the extract exhibits good antioxidant properties. The neuroprotective effects of the extract were investigated using PC12 cells. Under oxidative stress induced by H2O2, the extract dose-dependently protected the cells from the degenerative changes. The in vitro screening system was employed to identify the neuroprotective mechanisms of the extract. The results indicated that the extract protected PC12 cells against oxidative stress induced by H2O2 through its ability to reduce cell death by increasing cell viability and suppressing reactive oxygen species generation. Furthermore, the extract also inhibited the expression of alpha-syn and TH proteins, and the inhibitor of gene expression induced by alpha-syn and TH mRNA in the cells. These results suggest that the extract has neuroprotective properties and that it may act by inhibiting the accumulation and aggregation of alpha-syn and TH in neurons.// Copyright ©.NET Foundation and Contributors. All rights reserved. // Licensed under the MIT license. See LICENSE file in the project root for full license information. namespace PInvoke { using System; using System


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